News von SFB924

The groups of Corinna Dawid, Caroline Gutjahr and Martin Parniske wrote a review highlighting the literature known primary and secondary metabolites in Lotus japonicus.

Fri, 18 Aug 2023 07:24:45 +0200

Primary and Secondary Metabolites in Lotus japonicus. Journal of Agricultural and Food Chemistry, 71, 30, 11277–11303. doi: 10.1021/acs.jafc.3c02709 Josef L. Ranner, Sabrina Schalk, Cindy Martyniak, Martin Parniske, Caroline Gutjahr, Timo D. Stark, and Corinna Dawid (2023). Lotus japonicus is a leguminous model plant used to gain insight into plant physiology, stress response, and especially symbiotic plant–microbe interactions, such as root nodule symbiosis or arbuscular mycorrhiza. Responses to changing environmental conditions, stress, microbes, or insect pests are generally accompanied by changes in primary and secondary metabolism to account for physiological needs or to produce defensive or signaling compounds. Here we provide an overview of the primary and secondary metabolites identified in L. japonicus to date. Identification of the metabolites is mainly based on mass spectral tags (MSTs) obtained by gas chromatography linked with tandem mass spectrometry (GC–MS/MS) or liquid chromatography–MS/MS (LC–MS/MS). These MSTs contain retention index and mass spectral information, which are compared to databases with MSTs of authentic standards. More than 600 metabolites are grouped into compound classes such as polyphenols, carbohydrates, organic acids and phosphates, lipids, amino acids, nitrogenous compounds, phytohormones, and additional defense compounds. Their physiological effects are briefly discussed, and the detection methods are explained. This review of the exisiting literature on L. japonicus metabolites provides a valuable basis for future metabolomics studies.

The Vlot and Ranf labs describe functions of carbohydrate signaling in Arabidopsis immunity. The Vlot lab further used CRISPR-Cas genome-edited barley to reveal roles of systemic acquired resistance signals from Arabidopsis in this cereal crop.

Wed, 28 Jun 2023 08:51:45 +0200

Transfer from model to crop: the Vlot and Ranf labs describe functions of carbohydrate signaling in Arabidopsis immunity. The Vlot lab further used CRISPR-Cas genome-edited barley to reveal roles of systemic acquired resistance signals from Arabidopsis in this cereal crop. Kornelia Bauer, Shahran Nayem, Martin Lehmann, Marion Wenig, Lin-Jie Shu, Stefanie Ranf, Peter Geigenberger and A. Corina Vlot (2023). Beta-D-XYLOSIDASE 4 modulates systemic immune signaling in Arabidopsis thaliana. Front. Plant Sci. 13:1096800. doi: 10.3389/fpls.2022.1096800 Alessandro Brambilla, Miriam Lenk, Andrea Ghirardo, Laura Eccleston, Claudia Knappe, Baris Weber, Birgit Lange, Jafargholi Imani, Anton R. Schäffner, Jörg-Peter Schnitzler, and A. Corina Vlot (2023). Pippecolic acid synthesis is required for systemic acquired resistance and plant-to-plant-induced immunity in barley. Journal of Experimental Botany, Vol. 74, No. 10 pp. 3033–3046, 2023 doi.org/10.1093/jxb/erad095

SFB924 Hybrid seminar: Dr. Peng Yu, Universität Bonn (DE): "Genetic basis and environmental regulation of root development and its biotic interaction with the rhizosphere in maize."

Thu, 22 Jun 2023 13:06:00 +0200

Thur 22.06.2023 (17:15 h) - Hybrid seminar

Dr. Peng Yu (Universität Bonn, DE) Genetic basis and environmental regulation of root development and its biotic interaction with the rhizosphere in maize. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" HS12, Emil-Ramann-Straße 2, TUM-SoLS, Freising or: https://tum-conf.zoom.us/j/62755814405?pwd=akhJVjlqRTBhWlB6cFhSYzl3YUJaQT09
Meeting-ID: 627 5581 4405
Kenncode: 463022 (Host: Prof. Dr. Gerd Patrick Bienert, TUM-LS)

SFB 924 Hybrid seminar: Prof. Dr. Markus Schwarzländer, Westfälische Wilhelms-Universität Münster (DE): "Managing transitions in plant life through metabolic regulation & signalling at the subcellular level."

Thu, 25 May 2023 10:30:00 +0200

Thur 25.05.2023 (17:15 h) - Hybrid seminar

Prof. Dr. Markus Schwarzländer (Westfälische Wilhelms-Universität Münster, DE) Managing transitions in plant life through metabolic regulation & signalling at the subcellular level. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" HS12, Emil-Ramann-Straße 2, TUM-SoLS, Freising or Join Zoom Meeting
https://tum-conf.zoom.us/j/63876885934?pwd=ZE9tOFB1TkdCME1xbXg5Qkp1Q0l3QT09
Meeting-ID: 638 7688 5934
Kenncode: 276765 (Host: Prof. Dr. Kay Schneitz/Prof. Dr. Gerd Patrick Bienert, TUM-LS)

SFB924 Hybrid seminar: Dr. Julien Gronnier, ZMBP, Universität Tübingen (DE): "Spatial and temporal regulation of cell surface signaling: from the nanoscale to the organisms."

Thu, 27 Apr 2023 08:38:00 +0200

Thur 27.04.2023 (17:15 h) - Hybrid seminar

Dr. Julien Gronnier (ZMBP, Universität Tübingen, DE)

Spatial and temporal regulation of cell surface signaling: from the nanoscale to the organisms. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" HS12, Emil-Ramann-Straße 2, TUM-SoLS, Freising or: Join Zoom Meeting
Meeting ID:
Passcode: (Host: Prof. Dr. Kay Schneitz, TUM-LS)

SFB924 Hybrid seminar: Dr. Katharina Bürstenbinder, Leibniz-Institut für Pflanzenbiochemie Halle (DE): "Order in chaos: Orchestration of the plant cytoskeleton by intrisically disordered proteins."

Thu, 20 Apr 2023 09:15:00 +0200

Thur 20.04.2023 (17:15 h) - Hybrid seminar

Dr. Katharina Bürstenbinder (Leibniz-Institut für Pflanzenbiochemie Halle, DE)

Order in chaos: Orchestration of the plant cytoskeleton by intrisically disordered proteins. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" HS12, Emil-Ramann-Straße 2, TUM-SoLS, Freising or: Join Zoom Meeting
https://tum-conf.zoom.us/j/68147273166?pwd=SytTdk9uS2hOR2dCMTFmMU9xclFrQT09
Meeting ID: 681 4727 3166
Passcode: 837627 (Host: Prof. Dr. Ralph Hückelhoven / Prof. Dr. Claus Schwechheimer, TUM-LS)

The groups of Corinna Dawid and Ralph Hückelhoven show that after wounding, barley plants emit green leaf volatiles that induce hordatine accumulation and disease resistance in receiver plants.

Tue, 18 Apr 2023 07:48:12 +0200

Volatile-mediated signaling in barley induces metabolic reprogramming and resistance against the biotrophic fungus Blumeria hordei.

Plant Biol. 25: 72-84

S. Laupheimer, L. Kurzweil, R. Proels, S. B. Unsicker, T. D. Stark, C. Dawid, R. Hückelhoven (2023). Plants have evolved diverse secondary metabolites to counteract biotic stress. Volatile organic compounds (VOCs) are released upon herbivore attack or pathogen infection. Recent studies suggest that VOCs can act as signalling molecules in plant defence and induce resistance in distant organs and neighbouring plants. However, knowledge is lacking on the function of VOCs in biotrophic fungal infection on cereal plants.We analysed VOCs emitted by 13±1-day-old barley plants (Hordeum vulgare L.) after mechanical wounding using passive absorbers and TD-GC/MS. We investigated the effect of pure VOC and complex VOC mixtures released from wounded plants on the barley–powdery mildew interaction by pre-exposure in a dynamic headspace connected to a powdery mildew susceptibility assay. Untargeted metabolomics and lipidomics were applied to investigate metabolic changes in sender and receiver barley plants.Green leaf volatiles (GLVs) dominated the volatile profile of wounded barley plants, with (Z)-3-hexenyl acetate (Z3HAC) as the most abundant compound. Barley volatiles emitted after mechanical wounding enhanced resistance in receiver plants towards fungal infection. We found volatile-mediated modifications of the plant–pathogen interaction in a concentration-dependent manner. Pre-exposure with physiologically relevant concentrations of Z3HAC resulted in induced resistance, suggesting that this GLV is a key player in barley anti-pathogen defence.The complex VOC mixture released from wounded barley and Z3HAC induced e.g. accumulation of chlorophyll, linolenic acid and linolenate-conjugated lipids, as well as defence-related secondary metabolites, such as hordatines in receiving plants. Barley VOCs hence induce a complex physiological response and disease resistance in receiver plants.

New publication from the Hückelhoven lab: CRIB motif-containing barley RIC157 is a novel ROP scaffold protein that interacts directly with barley RACB, promotes susceptibility to fungal penetration, and colocalizes with RACB at the haustorial entry site.

Tue, 18 Apr 2023 07:45:27 +0200

Barley RIC157, a potential RACB scaffold protein, is involved in susceptibility to powdery mildew. Plant Mol. Biol. 111:329-344 Stefan Engelhardt, Adriana Trutzenberg, Michaela Kopischke, Katja Probst, Christopher McCollum, Johanna Hofer, Ralph Hückelhoven (2023).

Successful obligate pathogens benefit from host cellular processes. For the biotrophic ascomycete fungus Blumeria hordei (Bh) it has been shown that barley RACB, a small monomeric G-protein (ROP, Rho of plants), is required for full susceptibility to fungal penetration. The susceptibility function of RACB probably lies in its role in cell polarity, which may be co-opted by the pathogen for invasive ingrowth of its haustorium. However, how RACB supports fungal penetration success and which other host proteins coordinate this process is incompletely understood. RIC (ROP-Interactive and CRIB-(Cdc42/Rac Interactive Binding) motif-containing) proteins are considered scaffold proteins which can interact directly with ROPs via a conserved CRIB motif. Here we describe a previously uncharacterized barley RIC protein, RIC157, which can interact directly with RACB in planta. We show that, in the presence of constitutively activated RACB, RIC157 shows a localization at the cell periphery/plasma membrane, whereas it otherwise localizes to the cytoplasm. RIC157 appears to mutually stabilize the plasma membrane localization of the activated ROP. During fungal infection, RIC157 and RACB colocalize at the penetration site, particularly at the haustorial neck. Additionally, transiently overexpressed RIC157 renders barley epidermal cells more susceptible to fungal penetration. We discuss that RIC157 may promote fungal penetration into barley epidermal cells by operating probably downstream of activated RACB.

SFB924 Hybrid seminar: Dr. Martin Bayer, ZMBP, Universität Tübingen (DE): "MAP kinase signaling makes the difference - a lesson from the plant embryo."

Thu, 06 Apr 2023 12:08:00 +0200

Thur 06.04.2023 (17:15 h) - Hybrid seminar

Dr. Martin Bayer (ZMBP, Universität Tübingen, DE)

MAP kinase signaling makes the difference - a lesson from the plant embryo. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" HS12, Emil-Ramann-Straße 2, TUM-SoLS, Freising or: Join the zoom-meeting
https://tum-conf.zoom.us/j/65846187125
Meeting-ID: 658 4618 7125
Passcode: 215553 (Host: Prof. Dr. Kay Schneitz, TUM-LS)

SFB924 Zoom seminar: Dr. Mark Waters, The University of Western Australia (AUS): "Butenolide signalling in plants and bacteria."

Thu, 30 Mar 2023 12:12:00 +0200

Thur 30.03.2023 (11:00 h) - Zoom Dr. Mark Waters (The University of Western Australia, AUS)

Butenolide signalling in plants and bacteria. SFB924 Kolloquium "Molecular Mechanisms of Agronomic Traits" Join Zoom Meeting
https://tum-conf.zoom.us/j/67373060668?pwd=cU1DaXFYdzRSSG5vV1RxYzNRL3VxUT09
Meeting ID: 673 7306 0668
Passcode: 958835 (Host: Prof. Dr. Caroline Gutjahr, TUM-LS and MPI for Molecular Plant Physiology, Golm)