New Publication on the Analytics of Vitamin B6

As a co-enzyme for over 150 biochemical reactions, Vitamin B₆ influences a substantial portion of the human metabolic enzymatic activity. The metabolically active form of vitamin B₆ is the vitamer pyridoxal-5’-phosphate (PLP). Pyridoxal (PL) together with pyridoxine (PN) and pyridoxamine (PM) as well as the respective phosphates (PLP, PNP, PMP) make up the group of B₆-vitamers. Additionally, plants can contain up to 70 % of the total vitamin B₆ content as pyridoxin-glycoside (PNG).

In a recent publication, the authors Bachmann, Maurer and Rychlik report the development of a stable isotope dilution analysis (SIDA) LC-MS/MS method for the quantification of five B₆ vitamers in different vegetables, fruits and wheat flower.